Construct: shRNA TRCN0000220303

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181754.1-757s1c2, TRCN0000026829, NM_181754.1-757s1c1
DNA Barcode:
GCACATTCCAAGAGCTGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gpr141 (353346)
Gene Description:
G protein-coupled receptor 141
Transcript:
RefSeq NM_181754.1 (NON-CURRENT)
Match location:
Position 757 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220303)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACATTCCAAGAGCTGTAAA
Hairpin Sequence:
5'-CCGG-GCACATTCCAAGAGCTGTAAA-CTCGAG-TTTACAGCTCTTGGAATGTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACATTCCAAGAGCTGTAAACTCGAGTTTACAGCTCTTGGAATGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACATTCCAAGAGCTGTAAACTCGAGTTTACAGCTCTTGGAATGTGC-3'

Other clones with same target sequence:

(none)