Construct: shRNA TRCN0000220318

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010287.1-826s1c2, TRCN0000026837, NM_010287.1-826s1c1
DNA Barcode:
CCATCACCAAGGGTGTCATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gpr83 (14608)
Gene Description:
G protein-coupled receptor 83
Transcript:
RefSeq NM_010287.1 (NON-CURRENT)
Match location:
Position 826 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220318)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATCACCAAGGGTGTCATAT
Hairpin Sequence:
5'-CCGG-CCATCACCAAGGGTGTCATAT-CTCGAG-ATATGACACCCTTGGTGATGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATCACCAAGGGTGTCATATCTCGAGATATGACACCCTTGGTGATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATCACCAAGGGTGTCATATCTCGAGATATGACACCCTTGGTGATGG-3'

Other clones with same target sequence:

(none)