Construct: shRNA TRCN0000220332

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005771.3-2035s1c2, TRCN0000027313, NM_005771.3-2035s1c1
DNA Barcode:
CCATCCAAATATGCAGTGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DHRS9 (10170)
Gene Description:
dehydrogenase/reductase 9
Transcript:
RefSeq NM_005771.3 (NON-CURRENT)
Match location:
Position 2035 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220332)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATCCAAATATGCAGTGGAA
Hairpin Sequence:
5'-CCGG-CCATCCAAATATGCAGTGGAA-CTCGAG-TTCCACTGCATATTTGGATGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATCCAAATATGCAGTGGAACTCGAGTTCCACTGCATATTTGGATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATCCAAATATGCAGTGGAACTCGAGTTCCACTGCATATTTGGATGG-3'

Other clones with same target sequence:

(none)