Construct: shRNA TRCN0000220353

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_170697.1-425s1c2, TRCN0000027254, NM_170697.1-425s1c1
DNA Barcode:
GTGGCAATACAGTAGTTATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ALDH1A2 (8854)
Gene Description:
aldehyde dehydrogenase 1 family member A2
Transcript:
RefSeq NM_170697.1 (NON-CURRENT)
Match location:
Position 425 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220353)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGCAATACAGTAGTTATTA
Hairpin Sequence:
5'-CCGG-GTGGCAATACAGTAGTTATTA-CTCGAG-TAATAACTACTGTATTGCCAC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGCAATACAGTAGTTATTACTCGAGTAATAACTACTGTATTGCCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGCAATACAGTAGTTATTACTCGAGTAATAACTACTGTATTGCCAC-3'

Other clones with same target sequence:

  1. TRCN0000233274