Construct: shRNA TRCN0000220354
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_170697.1-178s1c2, TRCN0000027291, NM_170697.1-178s1c1
- DNA Barcode:
- GCAACCATGGAATCCCTAAAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ALDH1A2 (8854)
- Gene Description:
- aldehyde dehydrogenase 1 family member A2
- Transcript:
- RefSeq NM_170697.1 (NON-CURRENT)
- Match location:
- Position 178 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000220354)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCAACCATGGAATCCCTAAAT
- Hairpin Sequence:
-
5'-CCGG-GCAACCATGGAATCCCTAAAT-CTCGAG-ATTTAGGGATTCCATGGTTGC-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCAACCATGGAATCCCTAAATCTCGAGATTTAGGGATTCCATGGTTGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCAACCATGGAATCCCTAAATCTCGAGATTTAGGGATTCCATGGTTGC-3'
Other clones with same target sequence:
(none)
