Construct: shRNA TRCN0000220418

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011210.1-960s1c2, TRCN0000029932, NM_011210.1-960s1c1
DNA Barcode:
GCCTAATAATGTGACCAGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ptprc (19264)
Gene Description:
protein tyrosine phosphatase, receptor type, C
Transcript:
RefSeq NM_011210.1 (NON-CURRENT)
Match location:
Position 960 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220418)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTAATAATGTGACCAGTTT
Hairpin Sequence:
5'-CCGG-GCCTAATAATGTGACCAGTTT-CTCGAG-AAACTGGTCACATTATTAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTAATAATGTGACCAGTTTCTCGAGAAACTGGTCACATTATTAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTAATAATGTGACCAGTTTCTCGAGAAACTGGTCACATTATTAGGC-3'

Other clones with same target sequence:

(none)