Construct: shRNA TRCN0000220422

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016933.2-527s1c2, TRCN0000029936, NM_016933.2-527s1c1
DNA Barcode:
GCCCTGCTGAGTGACCTGCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ptprcap (19265)
Gene Description:
protein tyrosine phosphatase, receptor type, C polypeptide-associated protein
Transcript:
RefSeq NM_016933.2 (NON-CURRENT)
Match location:
Position 527 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220422)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTGCTGAGTGACCTGCAT
Hairpin Sequence:
5'-CCGG-GCCCTGCTGAGTGACCTGCAT-CTCGAG-ATGCAGGTCACTCAGCAGGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTGCTGAGTGACCTGCATCTCGAGATGCAGGTCACTCAGCAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTGCTGAGTGACCTGCATCTCGAGATGCAGGTCACTCAGCAGGGC-3'

Other clones with same target sequence:

(none)