Construct: shRNA TRCN0000220423

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011212.2-2524s1c2, TRCN0000029939, NM_011212.2-2524s1c1
DNA Barcode:
CCTTCCTAATTCCTTTGTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ptpre (19267)
Gene Description:
protein tyrosine phosphatase, receptor type, E
Transcript:
RefSeq NM_011212.2 (NON-CURRENT)
Match location:
Position 2524 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220423)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCCTAATTCCTTTGTATA
Hairpin Sequence:
5'-CCGG-CCTTCCTAATTCCTTTGTATA-CTCGAG-TATACAAAGGAATTAGGAAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCCTAATTCCTTTGTATACTCGAGTATACAAAGGAATTAGGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCCTAATTCCTTTGTATACTCGAGTATACAAAGGAATTAGGAAGG-3'

Other clones with same target sequence:

(none)