Construct: shRNA TRCN0000220435

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008981.1-2712s1c2, TRCN0000029951, NM_008981.1-2712s1c1
DNA Barcode:
CCTGTCAAACAGTTTGGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ptprg (19270)
Gene Description:
protein tyrosine phosphatase, receptor type, G
Transcript:
RefSeq NM_008981.1 (NON-CURRENT)
Match location:
Position 2712 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220435)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTCAAACAGTTTGGTAAA
Hairpin Sequence:
5'-CCGG-CCTGTCAAACAGTTTGGTAAA-CTCGAG-TTTACCAAACTGTTTGACAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTCAAACAGTTTGGTAAACTCGAGTTTACCAAACTGTTTGACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTCAAACAGTTTGGTAAACTCGAGTTTACCAAACTGTTTGACAGG-3'

Other clones with same target sequence:

(none)