Construct: shRNA TRCN0000220448

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011214.1-3243s1c2, TRCN0000029964, NM_011214.1-3243s1c1
DNA Barcode:
GCCTGAGATGATCTACGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ptpru (19273)
Gene Description:
protein tyrosine phosphatase, receptor type, U
Transcript:
RefSeq NM_011214.1 (NON-CURRENT)
Match location:
Position 3243 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220448)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGAGATGATCTACGATTT
Hairpin Sequence:
5'-CCGG-GCCTGAGATGATCTACGATTT-CTCGAG-AAATCGTAGATCATCTCAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGAGATGATCTACGATTTCTCGAGAAATCGTAGATCATCTCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGAGATGATCTACGATTTCTCGAGAAATCGTAGATCATCTCAGGC-3'

Other clones with same target sequence:

(none)