Construct: shRNA TRCN0000220488

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011877.1-2516s1c2, TRCN0000030005, NM_011877.1-2516s1c1
DNA Barcode:
CCGCTTTCTCTCAGGAACTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ptpn21 (24000)
Gene Description:
protein tyrosine phosphatase, non-receptor type 21
Transcript:
RefSeq NM_011877.1 (NON-CURRENT)
Match location:
Position 2516 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220488)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCTTTCTCTCAGGAACTAA
Hairpin Sequence:
5'-CCGG-CCGCTTTCTCTCAGGAACTAA-CTCGAG-TTAGTTCCTGAGAGAAAGCGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCTTTCTCTCAGGAACTAACTCGAGTTAGTTCCTGAGAGAAAGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCTTTCTCTCAGGAACTAACTCGAGTTAGTTCCTGAGAGAAAGCGG-3'

Other clones with same target sequence:

  1. TRCN0000293055