Construct: shRNA TRCN0000220490
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_011877.1-1114s1c2, TRCN0000030007, NM_011877.1-1114s1c1
- DNA Barcode:
- GCACTAGAGCTGGCAAATAAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Ptpn21 (24000)
- Gene Description:
- protein tyrosine phosphatase, non-receptor type 21
- Transcript:
- RefSeq NM_011877.1 (NON-CURRENT)
- Match location:
- Position 1114 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000220490)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCACTAGAGCTGGCAAATAAA
- Hairpin Sequence:
-
5'-CCGG-GCACTAGAGCTGGCAAATAAA-CTCGAG-TTTATTTGCCAGCTCTAGTGC-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCACTAGAGCTGGCAAATAAACTCGAGTTTATTTGCCAGCTCTAGTGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCACTAGAGCTGGCAAATAAACTCGAGTTTATTTGCCAGCTCTAGTGC-3'
Other clones with same target sequence:
-
TRCN0000293054
