Construct: shRNA TRCN0000220493

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011884.1-515s1c2, TRCN0000030011, NM_011884.1-515s1c1
DNA Barcode:
CCAGGAATCTACAAGGGTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rngtt (24018)
Gene Description:
RNA guanylyltransferase and 5'-phosphatase
Transcript:
RefSeq NM_011884.1 (NON-CURRENT)
Match location:
Position 515 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220493)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGAATCTACAAGGGTGAT
Hairpin Sequence:
5'-CCGG-CCAGGAATCTACAAGGGTGAT-CTCGAG-ATCACCCTTGTAGATTCCTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGAATCTACAAGGGTGATCTCGAGATCACCCTTGTAGATTCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGAATCTACAAGGGTGATCTCGAGATCACCCTTGTAGATTCCTGG-3'

Other clones with same target sequence:

(none)