Construct: shRNA TRCN0000220503

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153107.1-441s1c2, TRCN0000033172, NM_153107.1-441s1c1
DNA Barcode:
CTTCCTTGATTGTGCCCAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cpz (242939)
Gene Description:
carboxypeptidase Z
Transcript:
RefSeq NM_153107.1 (NON-CURRENT)
Match location:
Position 441 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220503)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCCTTGATTGTGCCCAATA
Hairpin Sequence:
5'-CCGG-CTTCCTTGATTGTGCCCAATA-CTCGAG-TATTGGGCACAATCAAGGAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCCTTGATTGTGCCCAATACTCGAGTATTGGGCACAATCAAGGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCCTTGATTGTGCCCAATACTCGAGTATTGGGCACAATCAAGGAAG-3'

Other clones with same target sequence:

(none)