Construct: shRNA TRCN0000220518

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025827.2-701s1c2, TRCN0000033188, NM_025827.2-701s1c1
DNA Barcode:
GATCGGTTCAAGATGACCATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lonp2 (66887)
Gene Description:
lon peptidase 2, peroxisomal
Transcript:
RefSeq NM_025827.2 (NON-CURRENT)
Match location:
Position 701 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220518)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCGGTTCAAGATGACCATA
Hairpin Sequence:
5'-CCGG-GATCGGTTCAAGATGACCATA-CTCGAG-TATGGTCATCTTGAACCGATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCGGTTCAAGATGACCATACTCGAGTATGGTCATCTTGAACCGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCGGTTCAAGATGACCATACTCGAGTATGGTCATCTTGAACCGATC-3'

Other clones with same target sequence:

  1. TRCN0000308493