Construct: shRNA TRCN0000220519

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008454.1-674s1c2, TRCN0000033189, NM_008454.1-674s1c1
DNA Barcode:
GCTGTGTACTACCACAAGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Klk1b16 (16615)
Gene Description:
kallikrein 1-related peptidase b16
Transcript:
RefSeq NM_008454.1 (NON-CURRENT)
Match location:
Position 674 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220519)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTGTACTACCACAAGGAA
Hairpin Sequence:
5'-CCGG-GCTGTGTACTACCACAAGGAA-CTCGAG-TTCCTTGTGGTAGTACACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTGTACTACCACAAGGAACTCGAGTTCCTTGTGGTAGTACACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTGTACTACCACAAGGAACTCGAGTTCCTTGTGGTAGTACACAGC-3'

Other clones with same target sequence:

(none)