Construct: shRNA TRCN0000220537

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175214.3-3382s1c2, TRCN0000033207, NM_175214.3-3382s1c1
DNA Barcode:
CGTAAACAACAGTTGCAGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kif27 (75050)
Gene Description:
kinesin family member 27
Transcript:
RefSeq NM_175214.3 (CURRENT)
Match location:
Position 3382 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220537)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTAAACAACAGTTGCAGAAT
Hairpin Sequence:
5'-CCGG-CGTAAACAACAGTTGCAGAAT-CTCGAG-ATTCTGCAACTGTTGTTTACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTAAACAACAGTTGCAGAATCTCGAGATTCTGCAACTGTTGTTTACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTAAACAACAGTTGCAGAATCTCGAGATTCTGCAACTGTTGTTTACG-3'

Other clones with same target sequence:

(none)