Construct: shRNA TRCN0000220547

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027604.2-2135s1c2, TRCN0000033217, NM_027604.2-2135s1c1
DNA Barcode:
GCGCTTGTTTACATTCCAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Usp15 (14479)
Gene Description:
ubiquitin specific peptidase 15
Transcript:
RefSeq NM_027604.2 (NON-CURRENT)
Match location:
Position 2135 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220547)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCTTGTTTACATTCCAGTT
Hairpin Sequence:
5'-CCGG-GCGCTTGTTTACATTCCAGTT-CTCGAG-AACTGGAATGTAAACAAGCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCTTGTTTACATTCCAGTTCTCGAGAACTGGAATGTAAACAAGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCTTGTTTACATTCCAGTTCTCGAGAACTGGAATGTAAACAAGCGC-3'

Other clones with same target sequence:

(none)