Construct: shRNA TRCN0000220592

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033225.3-5411s1c2, TRCN0000005992, NM_033225.3-5411s1c1
DNA Barcode:
CCCTGAATGATTTGGCAGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CSMD1 (64478)
Gene Description:
CUB and Sushi multiple domains 1
Transcript:
RefSeq NM_033225.3 (NON-CURRENT)
Match location:
Position 5411 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220592)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGAATGATTTGGCAGAAT
Hairpin Sequence:
5'-CCGG-CCCTGAATGATTTGGCAGAAT-CTCGAG-ATTCTGCCAAATCATTCAGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGAATGATTTGGCAGAATCTCGAGATTCTGCCAAATCATTCAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGAATGATTTGGCAGAATCTCGAGATTCTGCCAAATCATTCAGGG-3'

Other clones with same target sequence:

(none)