Construct: shRNA TRCN0000220597

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022650.1-1880s1c2, TRCN0000005997, NM_022650.1-1880s1c1
DNA Barcode:
GCACACTAAATGACAGAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RASA1 (5921)
Gene Description:
RAS p21 protein activator 1
Transcript:
RefSeq NM_022650.1 (NON-CURRENT)
Match location:
Position 1880 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220597)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACACTAAATGACAGAGAAA
Hairpin Sequence:
5'-CCGG-GCACACTAAATGACAGAGAAA-CTCGAG-TTTCTCTGTCATTTAGTGTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACACTAAATGACAGAGAAACTCGAGTTTCTCTGTCATTTAGTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACACTAAATGACAGAGAAACTCGAGTTTCTCTGTCATTTAGTGTGC-3'

Other clones with same target sequence:

(none)