Construct: shRNA TRCN0000220617

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003559.3-1693s1c2, TRCN0000006017, NM_003559.3-1693s1c1
DNA Barcode:
CAAACGCTTCAACGAGTTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIP4K2B (8396)
Gene Description:
phosphatidylinositol-5-phosphate 4-kinase type 2 beta
Transcript:
RefSeq NM_003559.3 (NON-CURRENT)
Match location:
Position 1693 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220617)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAACGCTTCAACGAGTTTAT
Hairpin Sequence:
5'-CCGG-CAAACGCTTCAACGAGTTTAT-CTCGAG-ATAAACTCGTTGAAGCGTTTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAACGCTTCAACGAGTTTATCTCGAGATAAACTCGTTGAAGCGTTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAACGCTTCAACGAGTTTATCTCGAGATAAACTCGTTGAAGCGTTTG-3'

Other clones with same target sequence:

(none)