Construct: shRNA TRCN0000220618
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_000476.1-242s1c2, TRCN0000006018, NM_000476.1-242s1c1
- DNA Barcode:
- GCTGTCGGAAATCATGGAGAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- AK1 (203)
- Gene Description:
- adenylate kinase 1
- Transcript:
- RefSeq NM_000476.1 (NON-CURRENT)
- Match location:
- Position 242 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000220618)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCTGTCGGAAATCATGGAGAA
- Hairpin Sequence:
-
5'-CCGG-GCTGTCGGAAATCATGGAGAA-CTCGAG-TTCTCCATGATTTCCGACAGC-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCTGTCGGAAATCATGGAGAACTCGAGTTCTCCATGATTTCCGACAGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCTGTCGGAAATCATGGAGAACTCGAGTTCTCCATGATTTCCGACAGC-3'
Other clones with same target sequence:
-
TRCN0000342694
