Construct: shRNA TRCN0000220628

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016282.2-731s1c2, TRCN0000006029, NM_016282.2-731s1c1
DNA Barcode:
CGGAACAGAAACCAACAAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AK3 (50808)
Gene Description:
adenylate kinase 3
Transcript:
RefSeq NM_016282.2 (NON-CURRENT)
Match location:
Position 731 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220628)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAACAGAAACCAACAAGAT
Hairpin Sequence:
5'-CCGG-CGGAACAGAAACCAACAAGAT-CTCGAG-ATCTTGTTGGTTTCTGTTCCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAACAGAAACCAACAAGATCTCGAGATCTTGTTGGTTTCTGTTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAACAGAAACCAACAAGATCTCGAGATCTTGTTGGTTTCTGTTCCG-3'

Other clones with same target sequence:

(none)