Construct: shRNA TRCN0000220658

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020990.2-1165s1c2, TRCN0000006059, NM_020990.2-1165s1c1
DNA Barcode:
GCTAAGCAAAGATAGCCGCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CKMT1B (1159)
Gene Description:
creatine kinase, mitochondrial 1B
Transcript:
RefSeq NM_020990.2 (NON-CURRENT)
Match location:
Position 1165 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220658)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAAGCAAAGATAGCCGCTT
Hairpin Sequence:
5'-CCGG-GCTAAGCAAAGATAGCCGCTT-CTCGAG-AAGCGGCTATCTTTGCTTAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAAGCAAAGATAGCCGCTTCTCGAGAAGCGGCTATCTTTGCTTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAAGCAAAGATAGCCGCTTCTCGAGAAGCGGCTATCTTTGCTTAGC-3'

Other clones with same target sequence:

(none)