Construct: shRNA TRCN0000220667

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010019.2-811s1c2, TRCN0000023994, NM_010019.2-811s1c1
DNA Barcode:
GCTGTGAGTTACGACTTTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dapk2 (13143)
Gene Description:
death-associated protein kinase 2
Transcript:
RefSeq NM_010019.2 (NON-CURRENT)
Match location:
Position 811 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220667)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTGAGTTACGACTTTGAT
Hairpin Sequence:
5'-CCGG-GCTGTGAGTTACGACTTTGAT-CTCGAG-ATCAAAGTCGTAACTCACAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTGAGTTACGACTTTGATCTCGAGATCAAAGTCGTAACTCACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTGAGTTACGACTTTGATCTCGAGATCAAAGTCGTAACTCACAGC-3'

Other clones with same target sequence:

(none)