Construct: shRNA TRCN0000220672

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_139298.4-1396s1c2, TRCN0000024000, XM_139298.4-1396s1c1
DNA Barcode:
GCAATCAAGCAGTTGGATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prkaa1 (105787)
Gene Description:
protein kinase, AMP-activated, alpha 1 catalytic subunit
Transcript:
RefSeq XM_139298.4 (NON-CURRENT)
Match location:
Position 1396 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220672)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAATCAAGCAGTTGGATTAT
Hairpin Sequence:
5'-CCGG-GCAATCAAGCAGTTGGATTAT-CTCGAG-ATAATCCAACTGCTTGATTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAATCAAGCAGTTGGATTATCTCGAGATAATCCAACTGCTTGATTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAATCAAGCAGTTGGATTATCTCGAGATAATCCAACTGCTTGATTGC-3'

Other clones with same target sequence:

(none)