Construct: shRNA TRCN0000220686

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080850.1-1619s1c2, TRCN0000024015, NM_080850.1-1619s1c1
DNA Barcode:
CCAGTCCTTAAAGAACATTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pask (269224)
Gene Description:
PAS domain containing serine/threonine kinase
Transcript:
RefSeq NM_080850.1 (NON-CURRENT)
Match location:
Position 1619 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220686)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTCCTTAAAGAACATTTA
Hairpin Sequence:
5'-CCGG-CCAGTCCTTAAAGAACATTTA-CTCGAG-TAAATGTTCTTTAAGGACTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTCCTTAAAGAACATTTACTCGAGTAAATGTTCTTTAAGGACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTCCTTAAAGAACATTTACTCGAGTAAATGTTCTTTAAGGACTGG-3'

Other clones with same target sequence:

(none)