Construct: shRNA TRCN0000220709

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139300.2-5523s1c2, TRCN0000024038, NM_139300.2-5523s1c1
DNA Barcode:
GCGGAGAAACTAGAGTCTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mylk (107589)
Gene Description:
myosin, light polypeptide kinase
Transcript:
RefSeq NM_139300.2 (NON-CURRENT)
Match location:
Position 5523 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220709)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGAGAAACTAGAGTCTGAA
Hairpin Sequence:
5'-CCGG-GCGGAGAAACTAGAGTCTGAA-CTCGAG-TTCAGACTCTAGTTTCTCCGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGAGAAACTAGAGTCTGAACTCGAGTTCAGACTCTAGTTTCTCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGAGAAACTAGAGTCTGAACTCGAGTTCAGACTCTAGTTTCTCCGC-3'

Other clones with same target sequence:

(none)