Construct: shRNA TRCN0000220746

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_196191.2-1434s1c2, TRCN0000025698, XM_196191.2-1434s1c1
DNA Barcode:
GCTGCTCTATCTGGCTTTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gpr152 (269053)
Gene Description:
G protein-coupled receptor 152
Transcript:
RefSeq XM_196191.2 (NON-CURRENT)
Match location:
Position 1434 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220746)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCTCTATCTGGCTTTCTT
Hairpin Sequence:
5'-CCGG-GCTGCTCTATCTGGCTTTCTT-CTCGAG-AAGAAAGCCAGATAGAGCAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCTCTATCTGGCTTTCTTCTCGAGAAGAAAGCCAGATAGAGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCTCTATCTGGCTTTCTTCTCGAGAAGAAAGCCAGATAGAGCAGC-3'

Other clones with same target sequence:

(none)