Construct: shRNA TRCN0000220750

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010130.1-2177s1c2, TRCN0000025782, NM_010130.1-2177s1c1
DNA Barcode:
CGGGATTCCTACACTATCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adgre1 (13733)
Gene Description:
adhesion G protein-coupled receptor E1
Transcript:
RefSeq NM_010130.1 (NON-CURRENT)
Match location:
Position 2177 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220750)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGATTCCTACACTATCTTT
Hairpin Sequence:
5'-CCGG-CGGGATTCCTACACTATCTTT-CTCGAG-AAAGATAGTGTAGGAATCCCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGATTCCTACACTATCTTTCTCGAGAAAGATAGTGTAGGAATCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGATTCCTACACTATCTTTCTCGAGAAAGATAGTGTAGGAATCCCG-3'

Other clones with same target sequence:

(none)