Construct: shRNA TRCN0000220757

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_359054.1-231s1c2, TRCN0000025781, XM_359054.1-231s1c1
DNA Barcode:
GCCCTTCAGTATTGTGTACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Htr1f (15557)
Gene Description:
5-hydroxytryptamine (serotonin) receptor 1F
Transcript:
RefSeq XM_359054.1 (NON-CURRENT)
Match location:
Position 231 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220757)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTTCAGTATTGTGTACAT
Hairpin Sequence:
5'-CCGG-GCCCTTCAGTATTGTGTACAT-CTCGAG-ATGTACACAATACTGAAGGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTTCAGTATTGTGTACATCTCGAGATGTACACAATACTGAAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTTCAGTATTGTGTACATCTCGAGATGTACACAATACTGAAGGGC-3'

Other clones with same target sequence:

(none)