Construct: shRNA TRCN0000220764

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011925.1-2270s1c2, TRCN0000025747, NM_011925.1-2270s1c1
DNA Barcode:
CCATAGCACATGGCTATCCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adgre5 (26364)
Gene Description:
adhesion G protein-coupled receptor E5
Transcript:
RefSeq NM_011925.1 (NON-CURRENT)
Match location:
Position 2270 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220764)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATAGCACATGGCTATCCTA
Hairpin Sequence:
5'-CCGG-CCATAGCACATGGCTATCCTA-CTCGAG-TAGGATAGCCATGTGCTATGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATAGCACATGGCTATCCTACTCGAGTAGGATAGCCATGTGCTATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATAGCACATGGCTATCCTACTCGAGTAGGATAGCCATGTGCTATGG-3'

Other clones with same target sequence:

(none)