Construct: shRNA TRCN0000220766

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011925.1-1416s1c2, TRCN0000025711, NM_011925.1-1416s1c1
DNA Barcode:
GTCACGTTCAAGTTCGACTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adgre5 (26364)
Gene Description:
adhesion G protein-coupled receptor E5
Transcript:
RefSeq NM_011925.1 (NON-CURRENT)
Match location:
Position 1416 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220766)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCACGTTCAAGTTCGACTTT
Hairpin Sequence:
5'-CCGG-GTCACGTTCAAGTTCGACTTT-CTCGAG-AAAGTCGAACTTGAACGTGAC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCACGTTCAAGTTCGACTTTCTCGAGAAAGTCGAACTTGAACGTGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCACGTTCAAGTTCGACTTTCTCGAGAAAGTCGAACTTGAACGTGAC-3'

Other clones with same target sequence:

(none)