Construct: shRNA TRCN0000220767

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011925.1-316s1c2, TRCN0000025735, NM_011925.1-316s1c1
DNA Barcode:
GCGTCTGTAACCTGGGATATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adgre5 (26364)
Gene Description:
adhesion G protein-coupled receptor E5
Transcript:
RefSeq NM_011925.1 (NON-CURRENT)
Match location:
Position 316 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220767)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTCTGTAACCTGGGATATA
Hairpin Sequence:
5'-CCGG-GCGTCTGTAACCTGGGATATA-CTCGAG-TATATCCCAGGTTACAGACGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTCTGTAACCTGGGATATACTCGAGTATATCCCAGGTTACAGACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTCTGTAACCTGGGATATACTCGAGTATATCCCAGGTTACAGACGC-3'

Other clones with same target sequence:

(none)