Construct: shRNA TRCN0000220796

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181850.1-2530s1c2, TRCN0000025739, NM_181850.1-2530s1c1
DNA Barcode:
GCGGGAAACAGTCATCCTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Grm3 (108069)
Gene Description:
glutamate receptor, metabotropic 3
Transcript:
RefSeq NM_181850.1 (NON-CURRENT)
Match location:
Position 2530 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220796)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGGAAACAGTCATCCTAAA
Hairpin Sequence:
5'-CCGG-GCGGGAAACAGTCATCCTAAA-CTCGAG-TTTAGGATGACTGTTTCCCGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGGAAACAGTCATCCTAAACTCGAGTTTAGGATGACTGTTTCCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGGAAACAGTCATCCTAAACTCGAGTTTAGGATGACTGTTTCCCGC-3'

Other clones with same target sequence:

(none)