Construct: shRNA TRCN0000220805

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016976.1-667s1c2, TRCN0000025702, NM_016976.1-667s1c1
DNA Barcode:
GCCATGTTCCACACATTAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Grm1 (14816)
Gene Description:
glutamate receptor, metabotropic 1
Transcript:
RefSeq NM_016976.1 (NON-CURRENT)
Match location:
Position 667 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220805)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATGTTCCACACATTAGAT
Hairpin Sequence:
5'-CCGG-GCCATGTTCCACACATTAGAT-CTCGAG-ATCTAATGTGTGGAACATGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATGTTCCACACATTAGATCTCGAGATCTAATGTGTGGAACATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATGTTCCACACATTAGATCTCGAGATCTAATGTGTGGAACATGGC-3'

Other clones with same target sequence:

(none)