Construct: shRNA TRCN0000220809

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008773.2-1496s1c2, TRCN0000025742, NM_008773.2-1496s1c1
DNA Barcode:
GCCTAACAGAACTGTGAGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
P2ry2 (18442)
Gene Description:
purinergic receptor P2Y, G-protein coupled 2
Transcript:
RefSeq NM_008773.2 (NON-CURRENT)
Match location:
Position 1496 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220809)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTAACAGAACTGTGAGGAA
Hairpin Sequence:
5'-CCGG-GCCTAACAGAACTGTGAGGAA-CTCGAG-TTCCTCACAGTTCTGTTAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTAACAGAACTGTGAGGAACTCGAGTTCCTCACAGTTCTGTTAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTAACAGAACTGTGAGGAACTCGAGTTCCTCACAGTTCTGTTAGGC-3'

Other clones with same target sequence:

(none)