Construct: shRNA TRCN0000220888

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000284.1-499s1c2, TRCN0000028630, NM_000284.1-499s1c1
DNA Barcode:
CGAGAAATTCTCGCAGAGCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDHA1 (5160)
Gene Description:
pyruvate dehydrogenase E1 alpha 1 subunit
Transcript:
RefSeq NM_000284.1 (NON-CURRENT)
Match location:
Position 499 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220888)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGAAATTCTCGCAGAGCTT
Hairpin Sequence:
5'-CCGG-CGAGAAATTCTCGCAGAGCTT-CTCGAG-AAGCTCTGCGAGAATTTCTCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGAAATTCTCGCAGAGCTTCTCGAGAAGCTCTGCGAGAATTTCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGAAATTCTCGCAGAGCTTCTCGAGAAGCTCTGCGAGAATTTCTCG-3'

Other clones with same target sequence:

(none)