Construct: shRNA TRCN0000220902

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152443.1-261s1c2, TRCN0000028605, NM_152443.1-261s1c1
DNA Barcode:
TGTAGAACAAATGTGCAGCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RDH12 (145226)
Gene Description:
retinol dehydrogenase 12
Transcript:
RefSeq NM_152443.1 (NON-CURRENT)
Match location:
Position 261 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220902)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTAGAACAAATGTGCAGCTT
Hairpin Sequence:
5'-CCGG-TGTAGAACAAATGTGCAGCTT-CTCGAG-AAGCTGCACATTTGTTCTACA-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTAGAACAAATGTGCAGCTTCTCGAGAAGCTGCACATTTGTTCTACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTAGAACAAATGTGCAGCTTCTCGAGAAGCTGCACATTTGTTCTACA-3'

Other clones with same target sequence:

(none)