Construct: shRNA TRCN0000220911

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144650.1-973s1c2, TRCN0000028598, NM_144650.1-973s1c1
DNA Barcode:
CCCAATTTCAGGTTTAGTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADHFE1 (137872)
Gene Description:
alcohol dehydrogenase iron containing 1
Transcript:
RefSeq NM_144650.1 (NON-CURRENT)
Match location:
Position 973 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220911)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAATTTCAGGTTTAGTGAA
Hairpin Sequence:
5'-CCGG-CCCAATTTCAGGTTTAGTGAA-CTCGAG-TTCACTAAACCTGAAATTGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAATTTCAGGTTTAGTGAACTCGAGTTCACTAAACCTGAAATTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAATTTCAGGTTTAGTGAACTCGAGTTCACTAAACCTGAAATTGGG-3'

Other clones with same target sequence:

(none)