Construct: shRNA TRCN0000220936

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181851.3-2039s1c2, TRCN0000030123, NM_181851.3-2039s1c1
DNA Barcode:
CCTGGAAGTACAAATCATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tpte (234129)
Gene Description:
transmembrane phosphatase with tensin homology
Transcript:
RefSeq NM_181851.3 (NON-CURRENT)
Match location:
Position 2039 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220936)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGAAGTACAAATCATAAT
Hairpin Sequence:
5'-CCGG-CCTGGAAGTACAAATCATAAT-CTCGAG-ATTATGATTTGTACTTCCAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGAAGTACAAATCATAATCTCGAGATTATGATTTGTACTTCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGAAGTACAAATCATAATCTCGAGATTATGATTTGTACTTCCAGG-3'

Other clones with same target sequence:

(none)