Construct: shRNA TRCN0000220938
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_146263.1-63s1c2, TRCN0000030125, NM_146263.1-63s1c1
- DNA Barcode:
- GCAGAGAGTTTGCTTTGTGAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- GA_x5J8B7W2BV0-3116-4045 (257663)
- Gene Description:
- olfactory receptor GA_x5J8B7W2BV0-3116-4045
- Transcript:
- RefSeq NM_146263.1 (NON-CURRENT)
- Match location:
- Position 63 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000220938)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCAGAGAGTTTGCTTTGTGAT
- Hairpin Sequence:
-
5'-CCGG-GCAGAGAGTTTGCTTTGTGAT-CTCGAG-ATCACAAAGCAAACTCTCTGC-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCAGAGAGTTTGCTTTGTGATCTCGAGATCACAAAGCAAACTCTCTGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCAGAGAGTTTGCTTTGTGATCTCGAGATCACAAAGCAAACTCTCTGC-3'
Other clones with same target sequence:
(none)
