Construct: shRNA TRCN0000220941

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146263.1-320s1c2, TRCN0000030128, NM_146263.1-320s1c1
DNA Barcode:
GCATTGAGATGTTCCTGCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
GA_x5J8B7W2BV0-3116-4045 (257663)
Gene Description:
olfactory receptor GA_x5J8B7W2BV0-3116-4045
Transcript:
RefSeq NM_146263.1 (NON-CURRENT)
Match location:
Position 320 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220941)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATTGAGATGTTCCTGCTTA
Hairpin Sequence:
5'-CCGG-GCATTGAGATGTTCCTGCTTA-CTCGAG-TAAGCAGGAACATCTCAATGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATTGAGATGTTCCTGCTTACTCGAGTAAGCAGGAACATCTCAATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATTGAGATGTTCCTGCTTACTCGAGTAAGCAGGAACATCTCAATGC-3'

Other clones with same target sequence:

(none)