Construct: shRNA TRCN0000221012

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207353.1-301s1c2, TRCN0000034162, NM_207353.1-301s1c1
DNA Barcode:
CCACTGGGTTCTGTATATGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UBE2E4P (286480)
Gene Description:
ubiquitin conjugating enzyme E2 E4 pseudogene
Transcript:
RefSeq NM_207353.1 (NON-CURRENT)
Match location:
Position 301 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221012)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTGGGTTCTGTATATGAA
Hairpin Sequence:
5'-CCGG-CCACTGGGTTCTGTATATGAA-CTCGAG-TTCATATACAGAACCCAGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTGGGTTCTGTATATGAACTCGAGTTCATATACAGAACCCAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTGGGTTCTGTATATGAACTCGAGTTCATATACAGAACCCAGTGG-3'

Other clones with same target sequence:

(none)