Construct: shRNA TRCN0000221070

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003300.2-1162s1c2, TRCN0000034221, NM_003300.2-1162s1c1
DNA Barcode:
CCTTCATTTACAGCGAGTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRAF3 (7187)
Gene Description:
TNF receptor associated factor 3
Transcript:
RefSeq NM_003300.2 (NON-CURRENT)
Match location:
Position 1162 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221070)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCATTTACAGCGAGTGAT
Hairpin Sequence:
5'-CCGG-CCTTCATTTACAGCGAGTGAT-CTCGAG-ATCACTCGCTGTAAATGAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCATTTACAGCGAGTGATCTCGAGATCACTCGCTGTAAATGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCATTTACAGCGAGTGATCTCGAGATCACTCGCTGTAAATGAAGG-3'

Other clones with same target sequence:

(none)