Construct: shRNA TRCN0000221077

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004210.3-577s1c2, TRCN0000034228, NM_004210.3-577s1c1
DNA Barcode:
GTTTGCCAATGAGGGCAACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NEURL1 (9148)
Gene Description:
neuralized E3 ubiquitin protein ligase 1
Transcript:
RefSeq NM_004210.3 (NON-CURRENT)
Match location:
Position 577 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221077)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTTGCCAATGAGGGCAACAT
Hairpin Sequence:
5'-CCGG-GTTTGCCAATGAGGGCAACAT-CTCGAG-ATGTTGCCCTCATTGGCAAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTTGCCAATGAGGGCAACATCTCGAGATGTTGCCCTCATTGGCAAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTTGCCAATGAGGGCAACATCTCGAGATGTTGCCCTCATTGGCAAAC-3'

Other clones with same target sequence:

(none)