Construct: shRNA TRCN0000221080

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006814.2-830s1c2, TRCN0000034232, NM_006814.2-830s1c1
DNA Barcode:
GCACTTATTGACCCTTCCTCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PSMF1 (9491)
Gene Description:
proteasome inhibitor subunit 1
Transcript:
RefSeq NM_006814.2 (NON-CURRENT)
Match location:
Position 830 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221080)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTTATTGACCCTTCCTCA
Hairpin Sequence:
5'-CCGG-GCACTTATTGACCCTTCCTCA-CTCGAG-TGAGGAAGGGTCAATAAGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTTATTGACCCTTCCTCACTCGAGTGAGGAAGGGTCAATAAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTTATTGACCCTTCCTCACTCGAGTGAGGAAGGGTCAATAAGTGC-3'

Other clones with same target sequence:

  1. TRCN0000333547