Construct: shRNA TRCN0000221084

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004899.3-463s1c2, TRCN0000034236, NM_004899.3-463s1c1
DNA Barcode:
CCCTCAGCTTTGCAGAATCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BABAM2 (9577)
Gene Description:
BRISC and BRCA1 A complex member 2
Transcript:
RefSeq NM_004899.3 (NON-CURRENT)
Match location:
Position 463 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221084)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCAGCTTTGCAGAATCTT
Hairpin Sequence:
5'-CCGG-CCCTCAGCTTTGCAGAATCTT-CTCGAG-AAGATTCTGCAAAGCTGAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCAGCTTTGCAGAATCTTCTCGAGAAGATTCTGCAAAGCTGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCAGCTTTGCAGAATCTTCTCGAGAAGATTCTGCAAAGCTGAGGG-3'

Other clones with same target sequence:

(none)