Construct: shRNA TRCN0000221118

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004951.2-285s1c2, TRCN0000011358, NM_004951.2-285s1c1
DNA Barcode:
GCCTACACGAATAGCCTACTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GPR183 (1880)
Gene Description:
G protein-coupled receptor 183
Transcript:
RefSeq NM_004951.2 (NON-CURRENT)
Match location:
Position 285 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221118)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTACACGAATAGCCTACTA
Hairpin Sequence:
5'-CCGG-GCCTACACGAATAGCCTACTA-CTCGAG-TAGTAGGCTATTCGTGTAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTACACGAATAGCCTACTACTCGAGTAGTAGGCTATTCGTGTAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTACACGAATAGCCTACTACTCGAGTAGTAGGCTATTCGTGTAGGC-3'

Other clones with same target sequence:

(none)