Construct: shRNA TRCN0000221124

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_057159.2-1297s1c2, TRCN0000011368, NM_057159.2-1297s1c1
DNA Barcode:
GCCTATGAGAAATTCTTCCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LPAR1 (1902)
Gene Description:
lysophosphatidic acid receptor 1
Transcript:
RefSeq NM_057159.2 (NON-CURRENT)
Match location:
Position 1297 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221124)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTATGAGAAATTCTTCCTT
Hairpin Sequence:
5'-CCGG-GCCTATGAGAAATTCTTCCTT-CTCGAG-AAGGAAGAATTTCTCATAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTATGAGAAATTCTTCCTTCTCGAGAAGGAAGAATTTCTCATAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTATGAGAAATTCTTCCTTCTCGAGAAGGAAGAATTTCTCATAGGC-3'

Other clones with same target sequence:

(none)